Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin
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منابع مشابه
Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
BACKGROUND Pseudomnas putida is a natural producer of medium chain length polyhydroxyalkanoates (mcl-PHA), a polymeric precursor of bioplastics. A two-fold increase of mcl-PHA production via inactivation of the glucose dehydrogenase gene gcd, limiting the metabolic flux towards side products like gluconate was achieved before. Here, we investigated the overproduction of enzymes catalyzing limit...
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para-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common preservatives in food, drugs, and personal care products, as well as high-performance bioplastics such as liquid crystal polymers. Pseudomonas putida KT2440 was engineered to produce PHBA from glucose via the shikimate pathway intermediate chorismate. To obtain the PHBA production strain, chorismate lyase Ubi...
متن کاملFunctional metagenomics using Pseudomonas putida expands the known diversity of polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate copolymers
Functional metagenomics using Pseudomonas putida expands the known diversity of 1 polyhydroxyalkanoate synthases and enables the production of novel polyhydroxyalkanoate 2 copolymers 3 4 14 15. CC-BY 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not. Abstract 17 Bacterially produced biodegradable pol...
متن کاملControlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440
The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of P...
متن کاملA reduction in growth rate of Pseudomonas putida KT2442 counteracts productivity advances in medium-chain-length polyhydroxyalkanoate production from gluconate
BACKGROUND The substitution of plastics based on fossil raw material by biodegradable plastics produced from renewable resources is of crucial importance in a context of oil scarcity and overflowing plastic landfills. One of the most promising organisms for the manufacturing of medium-chain-length polyhydroxyalkanoates (mcl-PHA) is Pseudomonas putida KT2440 which can accumulate large amounts of...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2020
ISSN: 1751-7915,1751-7915
DOI: 10.1111/1751-7915.13481